Interference with the Amyloclastic Determination of Serum Amylase
نویسندگان
چکیده
منابع مشابه
Serum amylase determination by Somogyi's amyloclastic method with use of a photometric end-point.
Somogyi’s amyloclastic amylase assay has been modified by substitution of a spectrophotometric end point for visual observation, thereby increasing precision without changing the Somogyi assay units. A method of estimating starch concentration from the intensity of the starchiodine color reaction is described; absorbance is directly proportional to starch concentration. Somogyi’s finding that c...
متن کاملPositive interference in Radioimmunoassay determination of serum total T3
Introduction: Determination of thyrotropin, total and free thyroxine and triiodothyronine are widely used as diagnostic methods for thyroid function evaluation. There have been numerous reports of interferences in thyroid hormone immunoassays. Herein, a prominent positive interference is reported for a radioimmunoassay kit of total triiodothyronine. Materials and Methods: In this study, a total...
متن کاملA rapid saccharogenic method for the determination of serum amylase.
In this procedure for serum amylase the sugars formed by enzymatic starch hydrolysis are quantitatively measured in a protein-free tungstic acid filtrate by the alkaline reduction of ferricyanide to ferrocyanide. The ferrocyanide formed is readily determined by measurement of the color produced with phosphomolybdic acid at room temperature. The reducing sugar formed, expressed as milligrams per...
متن کاملAmylase activity in serum and urine: comparison of results by the amyloclastic, dyed-starch, and nephelometric techniques.
We assayed serum and urine specimens for amylase activity by the nephelometric (I),dyed-starch (Amylochrome) (II), and mayloclastic (III) techniques. For serum, the correlation coefficients of the regression lines were: I vs. II, 0.978 (n = 106); I vs. III, 0.736 (n = 110); and II vs. III, 0.739 (n = 108). For urine, they were I vs. II, 0.938 (n = 49); and I vs. III, 0.752 (n = 46). Because cal...
متن کاملNew chromogenic substrate for determination of serum amylase activity.
A rapid assay for serum amylase activity has been developed based on the use of a new chromogenic substrate-Cibachron Blue-amylose. The procedure requires 0.1 ml of serum and measures the production of soluble chro. mogen, the formation of which islinearwith enzyme activity. Normal human serum has a mean amylase activity of 118 ± 37 (SD) mg dye/100 ml/15 mm or, in international units, 78 ± 24 (...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1973
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/19.6.686